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A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
34269 45016 2015 7 PDF Available
Title
A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme
Abstract

•Cryptococcus flavus GB-1 produces CfCLE that efficiently degrades biodegradable plastics.•The CfCLE-encoding gene, CfCLE1 was obtained and sequenced.•A CfCLE productivity enhanced mutant C. flavus GB-1-DMC1 was obtained.•Xylose-fed batch cultivation using a jar fermentor was useful to scale-up CfCLE production.•CfCLE degraded various BPs cast films.

The yeast strain, Cryptococcus flavus GB-1, isolated from rice husks as a candidate producer of a biodegradable plastic (BP)-degrading enzyme. The genomic DNA sequence encoding the BP-degrading enzyme of GB-1 was determined. The enzyme was named CfCLE because the deduced amino acid sequence (239 amino acids) was found to be 93% identical to that of CLE of Cryptococcus sp. S-2 (accession No. BAK82405.1). The strain GB-1 forms a clear zone (halo) around its colonies on emulsified polybutylene succinate-co-adipate (PBSA) agar plates containing soybean oil but not on plates containing glucose. A UV-induced mutant from this strain, designated as GB-1-DMC1, formed halos on emulsified PBSA plates containing glucose and showed 1.53 U/ml of PBSA-degrading activity in liquid culture with glucose, which was more than 2.5-fold higher than that of the parent strain GB-1 (0.58 U/ml). Moreover, using xylose as a carbon source enhanced the PBSA-degrading activity of both strains. Xylose fed-batch cultivation of GB-1 and GB-1-DMC1 using a 5-l jar fermentor for 72 h produced 12.1 and 21.2 U/ml of CfCLE, respectively. Purified CfCLE has a molecular mass of 22 kDa, and its optimal pH and temperature for enzyme activity is 7.8 and 45 °C, respectively. CfCLE degraded various BP cast films.

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Keywords
Biodegradable plastic; Cutinase-like enzyme; Cryptococcus flavus; Mutant; Jar fermentor
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A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1718–1724
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us