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Proteomic analysis of homocholine catabolic pathway in Pseudomonas sp. strain A9

Paper ID Volume ID Publish Year Pages File Format Full-Text
34272 45016 2015 13 PDF Available
Title
Proteomic analysis of homocholine catabolic pathway in Pseudomonas sp. strain A9
Abstract

•Homocholine resemble choline in many aspects of cholinergic metabolisms.•Recently there are increased trials of homocholine containing synthetic drugs.•This first proteomic study elucidates the catabolic pathway of homocholine.•This work allows better understanding of microbial catabolism of homocholine.

Homocholine, a quaternary ammonium compound that resembles choline in many cholinergic metabolisms, is recently incorporated in many synthetic pharmaceuticals drugs targeting various types of cancer and bone marrow cells. Here, we used a proteomic approach (two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF-MS) to elucidate profoundly the degradation pathway of homocholine by Pseudomonas sp. strain A9. Eighty-nine differentially abundant proteins were identified and were found to cover a broad range of biological processes and cellular responses of strain A9 to homocholine. Of them, most of the proteins involved in the direct uptake and catabolism of homocholine were identified. These results demonstrated that strain A9 uptake homocholine from the media with different transporting systems and then consequently catabolize it to acetyl-CoA and trimethylamine (TMA) by five enzymes namely homocholine dehydrogenase, β-alaninebetaine aldehyde dehydrogenase, β-alaninebetaine CoA transferase, 3-hydroxypropionate dehydrogenase, and methylmalonate semialdehyde dehydrogenase. The acetyl-CoA enters the TCA and glyoxylate pathways to generate energy and provide metabolic precursors for other pathways while TMA exclude out of the bacterial cells raising some environmental and health concerns. Overall, the findings of this study provide a first insight into the global proteomic changes occurring in the strain A9 cells under exposure to homocholine in the environment.

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Keywords
Beta-alanine betaine; DddD enzyme; 2-DE; Homocholine; Proteomic
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Proteomic analysis of homocholine catabolic pathway in Pseudomonas sp. strain A9
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1735–1747
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us