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Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
34277 45016 2015 5 PDF Available
Title
Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis
Abstract

•A DNA shuffling library was constructed from four feruloyl esterases.•Chimeras with enhanced thermal stability were identified.•Half-life of the best chimera increased to 22-fold of the most stable parental FAE.•Release of ferulic acid increases to 3.6–12.8-fold using the best chimera.

Feruloyl esterases (FAE) with high thermostability are greatly desired for efficient and economical industrial processes. A DNA shuffling strategy was applied to generate a chimeric library from four homologous FAEs originated from fungi. Chimeras with enhanced thermal stability compared to the parental FAEs were identified, which contained segments from 3 or 4 of the parental enzymes. After further incorporation of 2–4 additional beneficial mutations, the half-lives of inactivation of the chimeras at 65 °C were increased by up to 22 fold when compared to the most stable parental FAE. As a result, the release of ferulic acid from steam-exploded corn stalk using the best chimera was enhanced by 12.8-fold when compared to the best parental FAE.

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Keywords
Feruloyl esterase; Thermostability; DNA shuffling; Pichia pastoris
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Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1783–1787
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us