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A high-throughput sequencing study of bacterial communities in an autohydrogenotrophic denitrifying bio-ceramsite reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
34291 45016 2015 7 PDF Available
Title
A high-throughput sequencing study of bacterial communities in an autohydrogenotrophic denitrifying bio-ceramsite reactor
Abstract

•The highest NO3−-N and TN removal efficiencies reached 97.41% and 93.93%.•Pyrosequencing of 16S rRNA gene amplicons was used to determine bacterial community.•Genus Acinetobacter was the dominant contributor for nitrate removal.

For the first time, high-throughput sequencing was employed to investigate the microbial communities of the biofilm in an autohydrogenotrophic denitrifying bio-ceramsite reactor. 30,418 and 39,178 bacterial 16S rRNA gene sequences were obtained from biofilm Samples C1 and C2 in the reactor under HRT 16 h, pH 7.0 and HRT 48 h, pH 9.0 conditions. Results showed that the mainly reported autohydrogenotrophic denitrifying phyla Proteobacteria, Firmicutes and classes Gammaproteobacteria, Alphaproteobacteria, Bacilli were all detected in the reactor, and their high relative abundances demonstrated they played key roles in the autohydrogenotrophic denitrification process, suggesting that this bio-ceramsite reactor presented better denitrification performance. The heatmap analysis illustrated that the largest genus in Sample C1 was Acinetobacter, while Planomicrobium was the largest genus in Sample C2. In addition, the reported hydrogenotrophic denitrifying genera Ochrobactrum, Paracoccus, Pseudomonas, Hydrogenophaga, and Thauera were always observed in the reactor, suggesting that this bio-ceramsite reactor exhibited autohydrogenotrophic denitrifying capacity to convert nitrate to nitrogen gas. This work might add some new insights into microbial communities in autohydrogenotrophic denitrification process.

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Keywords
High-throughput sequencing; Autohydrogenotrophic; Bio-ceramsite; Phyla; Heatmap
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A high-throughput sequencing study of bacterial communities in an autohydrogenotrophic denitrifying bio-ceramsite reactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1904–1910
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us