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Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation

Paper ID Volume ID Publish Year Pages File Format Full-Text
34292 45016 2015 12 PDF Available
Title
Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation
Abstract

•Novel Ochrobactrum intermedium strain produced glycolipopeptidal biosurfactant.•The lipopeptide exhibited a high level of thermal, saline and pH stability.•The lipopeptide enhanced the degradation of longer chain n-alkanes and PAHs.•The Ochrobactrum intermedium strain effectively degraded petroleum sludge.•The strain produced stable emulsion while growing on the sludge.

The low water solubility of oil sludge and other petroleum hydrocarbon compounds is a major problem for their bioremediation. In this paper biosurfactant enhanced bioaviabability and subsequent degradation of oily sludge was investigated for that purpose, an efficient biosurfactant producing and oil sludge degrading bacterial strain CN3, identified as Ochrobactrum intermedium according to 16S rRNA gene sequencing, was isolated from wood treatment plant soil. The biosurfactant exhibited a high level of thermal stability, tolerance to extreme levels of salinity and a positive effect for increasing pH. The biosurfactant was identified as glycolipopeptidal after Fourier Transform Infrared (FT-IR) spectrometry, Thin Layer Chromatography (TLC), liquid chromatography/tandem mass spectrometry (LC–MS/MS) and biochemical compiosition analysis. The ability of the isolate to degrade petroleum sludge and the effect of its biosurfactant on biodegradation enhancement was tested in liquid culture experiments. The isolate degraded up to 40% of the hydrophobic long chain aliphatic and polycyclic aromatic hydrocarbons in liquid cultures with 4% (v/v) crude oil sludge while the biosurfactant amended microcosm showed up to 70% degradation of the most hydrophobic components of the petroleum sludge in 3 weeks. This suggests the potential application of the isolate and its biosurfactant in bioremediation of contaminated environment and microbial enhanced oil recovery.

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Keywords
Bioremediation; n-Alkanes; Lipopeptide; Ochrobactrum intermedium; Petroleum sludge; PAHs
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Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1911–1922
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us