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Regulators essential for nicotine degradation in Shinella sp. HZN7

Paper ID Volume ID Publish Year Pages File Format Full-Text
34296 45016 2015 4 PDF Available
Title
Regulators essential for nicotine degradation in Shinella sp. HZN7
Abstract

•A mutant of Shinella sp. HZN7 which failed to degrade nicotine was screened.•The two mutant genes composed a novel two-component regulatory system.•The two genes were both essential for nicotine degradation.

Shinella sp. HZN7 can degrade nicotine via a variant of the pyridine and pyrrolidine pathways. In this study, a transposon mutant N7-W13 that was unable to degrade nicotine was identified and the mutant genes were cloned and sequenced. The predicted gene products ORFW13-1 and ORFW13-2 showed similarities to transcriptional regulatory proteins and sensor histidine kinases, respectively, which are constituents of typical two-component regulator systems. The orfW13-1 gene was interrupted by a transposon insertion in the strain N7-W13, while we constructed a strain with an orfW13-2 gene disruption, known as N7ΔorfW13-2. The mutants N7-W13 and N7ΔorfW13-2 all lost the ability to degrade nicotine, but were still capable of metabolizing 6-hydroxy-nicotine. These results suggest that the regulators are essential for nicotine degradation in Shinella sp. HZN7.

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Keywords
Nicotine; Degradation; Two-component regulatory system; Shinella sp. HZN7
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Regulators essential for nicotine degradation in Shinella sp. HZN7
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1947–1950
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us