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Sugaring-out extraction of 2,3-butanediol from fermentation broths

Paper ID Volume ID Publish Year Pages File Format Full-Text
34297 45016 1957 7 PDF Available
Title
Sugaring-out extraction of 2,3-butanediol from fermentation broths
Abstract

•Sugaring-out extraction of 2,3-butanediol from fermentation broth was studied.•Most of 2,3-butanediol was distributed to top phase and glucose to bottom phase.•The glucose-rich bottom phase was reused for fermentation of 2,3-butanediol.•The phenomenon in the bottom phase-based medium was similar to that in the normal.

Sugaring-out is a novel phase separation method which was found firstly in the separation of acetonitrile. Nowadays sugaring-out extraction was tried in the recovery of metal ions, biomolecules, drugs and proteins, while its application in separation of hydrophilic primary metabolites from fermentation broths was unknown. In this work sugaring-out extraction of 2,3-butanediol was explored. The effects of sugars and solvents on phase separation and 2,3-butanediol distribution were investigated to obtain an appropriate sugaring-out extraction system. The system consisting of t-butanol/glucose/water was chosen to obtain the operation conditions according to 2,3-butanediol distribution and recovery, including glucose addition, 2,3-butanediol concentration and (NH4)2HPO4 concentration. The glucose-rich bottom phase was reused for fermentation of 2,3-butanediol after removing the solvent. When the fermentation broth (60.3 g/L 2,3-butanediol) was mixed with 30% (w/v) glucose, 1.8% (w/v) (NH4)2HPO4 and equal volume of t-butanol, 76.3% 2,3-butanediol was distributed into the top phase and 80.4% glucose into the bottom phase, and 78.0% soluble proteins and 86.8% lactic acid were separated from 2,3-butanediol. When the bottom phase was diluted to prepare fermentation medium, glucose consumption and 2,3-butanediol production were similar to those using freshly prepared normal fermentation medium. Sugaring-out extraction is a novel alternative separation for bio-based chemicals.

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Keywords
2,3-Butanediol; Sugaring-out extraction; Fermentation; Separation
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Sugaring-out extraction of 2,3-butanediol from fermentation broths
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1951–1957
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us