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Kaempferol mediated synthesis of gold nanoparticles and their cytotoxic effects on MCF-7 cancer cell line

Paper ID Volume ID Publish Year Pages File Format Full-Text
34299 45016 1976 11 PDF Available
Title
Kaempferol mediated synthesis of gold nanoparticles and their cytotoxic effects on MCF-7 cancer cell line
Abstract

•Synthesis of gold nanoparticles using kaempferol.•k-AuNPs possess anti-oxidant and anti-angiogenic activity.•Induction of cell death of MCF-7 cell line indicate anti-cancer activity.

Kaempferol (3,5,7,4′tetrahydroxyflavone), a flavonol present in a variety of plant sources, has been used for the formation of gold nanoparticles (k-AuNPs) and these particles have been evaluated for their anti-cancer properties. These nanoparticles have been formed at room temperature (RT) without any other stabilizing agents and are studied for the first time. The size of k-AuNPs is observed to be 16.5 ± 2.5 nm through high-resolution transmission electron microscopy (HR-TEM). The k-AuNPs are found to be hemocompatible and have anti-oxidant properties which have been evaluated using hemolysis assay and DPPH radical scavenging assay respectively. In the presence of k-AuNPs, there is a dose and time dependent decrease in viability of MCF-7 breast cancer cells which has been confirmed using ethidium bromide–acridine orange (EtBr–AO) staining, phase contrast microscopy (PCM) observations, and scanning electron microscopy (SEM). The anti-proliferation activity of k-AuNPs was assessed using CyQuant assay. The k-AuNPs induce apoptosis of the MCF-7 cells as evidenced through increase in the sub-G1 (hypodiploid) population. They also inhibit the vascular endothelial growth factor (VEGF) induced angiogenesis as demonstrated through chorioallantoic membrane assay (CAM). These results suggest the potential of k-AuNPs as a promising anti-cancer agent in breast cancer therapy.

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Keywords
Gold nanoparticles; Spectroscopy; HR-TEM; Apoptosis; Breast cancer; Kaempferol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1966–1976
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us