Stalked sea squirt (Styela clava) tunic waste as a valuable bioresource: Cosmetic and antioxidant activities
•SCT extracts show strong inhibitory effects on skin aging-related enzymes.•SCT extracts show lower IC50 values than ketoprofen, a representative hyaluronidase inhibitor.•SCT extracts show strong in vitro antioxidant activity.•This is the first report on the antiaging potential of SCT.
The aim of this study was to assess the skin aging related-enzyme inhibitory effects and antioxidant effects of different solvent extracts of the tunics of the solitary ascidian tunicate Styela clava, which are regarded as fishery waste. All extracts dose-dependently inhibited the enzyme activities of collagenase, elastase and hyaluronidase. The anticollagenase activities (IC50) of hot water (5.058 mg/ml) and n-butanol (5.760 mg/ml) extracts were comparable to that of ascorbic acid (3.838 mg/ml), but lower than that of EDTA (10.856 mg/ml). The antielastase activities (IC50) of ethanol (0.356 mg/ml), acetone (1.668 mg/ml), and n-butanol (2.040 mg/ml) extracts were much lower than that of ursolic acid (4.073 mg/ml), a representative elastase inhibitor, and the antihyaluronidase activities of ethyl acetate (0.210 mg/ml) and hexane (0.596 mg/ml) extracts were lower than that of ketoprofen, a well-known hyaluronidase inhibitor. Hot water extract scavenged ABTS radicals with an IC50 value of 0.192 mg/ml, which was comparable to that of ascorbic acid (0.052 mg/ml). These results suggest that S. clava tunics (SCTs) are a potential candidate for wrinkle-care cosmetics and for the treatment of inflammation-related disorders. As far as we are aware, this is the first report to describe the potential antiaging activity of SCT.
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Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1977–1984