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Two-stage repeated-batch fermentation of immobilized Clostridium beijerinckii on oil palm fronds for solvents production

Paper ID Volume ID Publish Year Pages File Format Full-Text
34303 45017 2015 10 PDF Available
Title
Two-stage repeated-batch fermentation of immobilized Clostridium beijerinckii on oil palm fronds for solvents production
Abstract

•Oil palm fronds (OPF) are potential support materials for clostridium immobilization.•Immobilized cells on OPF produced high butanol in repeated-batch fermentation.•Two-stage repeated-batch fermentation improved solvent productivity.

In this study, palm tree wastes including oil palm shells, palm press fibers, empty fruit bunches and oil palm fronds were evaluated as possible eco-friendly support materials for immobilization of solvent-producing Clostridium beijerinckii. Among support materials tested, oil palm fronds are the most suitable support materials for cell immobilization and repeated-batch production of solvents by non-growing cells incubated in nitrogen-free medium. The non-growing immobilized cells on 7% (w/v) oil palm fronds produced the highest solvent yield of 12.2 g/L in the 1st batch. However, in the 2nd and 3rd batches the solvent production was reduced to 9.01 g/L and 4.95 g/L, respectively. Two-stage repeated-batch fermentation in which immobilized cells were re-incubated in nitrogen-rich medium (Stage I) before being re-used in nitrogen-free medium (Stage II), were then attempted. The solvents produced by this strategy remained >80% in the 3rd cycle and >55% in the 4th cycle. The total solvent yields in the 1st, 2nd, 3rd and 4th cycles were as high as 31.5, 30.6, 25.3 and 17.5 g/L, respectively. This study has shown that lignocellulosic palm tree wastes can be used as low-cost support materials for immobilization of clostridia and the solvent productivity can be improved by two-stage repeated-batch fermentation.

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Keywords
Butanol; Immobilization; Clostridium beijerinckii; Oil palm fronds; Repeated-batch fermentation
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Two-stage repeated-batch fermentation of immobilized Clostridium beijerinckii on oil palm fronds for solvents production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 8, August 2015, Pages 1167–1176
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us