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Laccase production by newly isolated white rot fungus Funalia trogii: Effect of immobilization matrix on laccase production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3431 170 2013 6 PDF Available
Title
Laccase production by newly isolated white rot fungus Funalia trogii: Effect of immobilization matrix on laccase production
Abstract

The laccase production performance of newly isolated Funalia trogii (F. trogii) immobilized on low-cost activated adsorbents prepared from apricot stone was investigated in inexpensive molasses medium and compared with free pellets. The adsorbents were apricot stone-based activated carbon (AC), Cu-impregnated apricot stone-based activated carbon (Cu–AC) and Fe-impregnated apricot stone-based activated carbon (Fe–AC). Cu–AC and Fe–AC were prepared by chemical activation. The values of BET surface area (SBET), total pore volume (Vt), micropore surface area (Smic) and micropore volume (Vmic) which show the pore properties of the activated carbons were 133 m2 g−1, 0.105 cm3 g−1, 108 m2 g−1 and 0.056 cm3 g−1 for Cu–AC and 145 m2 g−1, 0.112 cm3 g−1, 117 m2 g−1 and 0.061 cm3 g−1 for Fe–AC, respectively. F. trogii attached well on all of these adsorbents. However, the fungus immobilized on Cu–AC produced much higher levels of laccase than the others. The laccase activity obtained in 5 g L−1 molasses media after first cycle and mean laccase activity obtained after three cycles with fungus immobilized on Cu–AC were 29.23 U mL−1 and 27.04 U mL−1, respectively. These activities were only 0.25 and 0.39 U mL−1 with free pellets. The crude laccase from immobilized culture could also decolorize the textile dyes.

► We prepared the low-cost activated adsorbents from apricot stone. ► The Cu–AC was firstly used for immobilization of pellets and for laccase production. ► The fungus immobilized on Cu–AC produced high levels of laccase. ► The crude laccase from immobilized culture decolorized the textile dyes.

Keywords
Activated carbon; Enzymes; Enzyme production; Fermentation; Filamentous fungi; Laccase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 71, 15 February 2013, Pages 134–139
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us