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Heterologous β-glucosidase in a fungal cellulase system: Comparison of different methods for development of multienzyme cocktails

Paper ID Volume ID Publish Year Pages File Format Full-Text
34313 45017 2015 6 PDF Available
Title
Heterologous β-glucosidase in a fungal cellulase system: Comparison of different methods for development of multienzyme cocktails
Abstract

•Aspergillus niger β-glucosidase (AnBGL) boosts hydrolytic performance of cellulases.•Cofermentation of cellulase and AnBGL-expressing Penicillium strains was carried out.•113% increase in glucose yield in Avicel hydrolysis was achieved using this approach.•Cofermentation of two strains of the same fungus is promising.

Three approaches for enrichment of Penicillium verruculosum cellulase multienzyme cocktails with a heterologously expressed Aspergillus niger β-glucosidase (AnBGL) for boosting the hydrolytic performance of cellulases were tested. The best boosting effects (73–113%) on glucose yield in 72-h hydrolysis of milled aspen wood and Avicel provided the enzyme preparation obtained by cofermentation of two P. verruculosum strains, one of which was a basic high-cellulase strain, while the other was capable to produce the heterologously expressed AnBGL (up to 80% of the total protein when used in a separate fermentation). Two other approaches, based on individual fermentations of P. verruculosum strains, carrying the heterologous AnBGL under the control of either a strong cbh1 gene promoter or a weaker hist4 gene promoter, also provided notable boosting effects, although they were less pronounced than in the first mentioned case.

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Keywords
β-Glucosidase; Cellulase; Heterologous expression; Penicillium verruculosum
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Heterologous β-glucosidase in a fungal cellulase system: Comparison of different methods for development of multienzyme cocktails
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 8, August 2015, Pages 1258–1263
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us