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Native-state pea albumin and globulin behavior upon transglutaminase treatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
34317 45017 2015 9 PDF Available
Title
Native-state pea albumin and globulin behavior upon transglutaminase treatment
Abstract

•MTGase thermal and pH-stability were determined for 5 h.•Optimum MTGase reaction conditions occur at pH 7, 40 °C, 20 mM NaCl for 2 h.•Pea globulin at native state is moderately good substrate to MTGase.•Pea albumin at native state is poor substrate to MTGase.

The behavior of pea albumin (Alb) and globulin (Glob) in their native state upon microbial transglutaminase (MTGase) treatment was studied. Only Glob was able to form a gel, at up to a 10% (w/w) concentration, with a minimum gelling concentration of 6% (w/w), and with a cross-linking degree of 25%. The most affected Glob subunits were convicilin (71 kDa), vicilins (55, 50, and 35 kDa), and legumin acidic subunit (40 kDa). In contrast, the legumin basic subunit (20 kDa) and vicilins of molecular weight less than 20 kDa remained mostly intact in all studied conditions. The cross-linking degree of Alb was 12%, which was not sufficient to form MTGase-induced gel. Major albumin polypeptide (PA2 26 kDa) was not affected by the MTGase concentration or by pH variation. Pea Alb and Glob in their native state were ranked as poor and moderately good substrates for MTGase, respectively, and unfolding them by thermal or chemical denaturation could be an interesting way to improve the efficiency of cross-linking.

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Keywords
Pea albumins; Pea globulins; Microbial transglutaminase properties; Cross-linking degree; Optimum parameters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 8, August 2015, Pages 1284–1292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us