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Highly efficient and regioselective acylation of arbutin catalyzed by lipase from Candida sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
34336 45018 2015 4 PDF Available
Title
Highly efficient and regioselective acylation of arbutin catalyzed by lipase from Candida sp.
Abstract

•CSL was firstly used for the acylation of arbutin.•Optimum conditions were screened for obtaining highest enzyme activity.•CSL exhibits highly regioselective specific (>99%) for the acylation of arbutin.

Arbutin, as a simple polyphenol, can be extracted from almost all the natural plants and has various important bioactivities. Regioselective acylation of arbutin can improve its cell membrane penetration and enhance its biological activity. The regioselective acylation of arbutin catalyzed by lipase from Candida sp. (CSL, expressed from Canadia sp. 99–125) has been successfully conducted in non-aqueous media. Under the optimum conditions (arbutin, 0.73 mmol; vinyl acetate, 29.71 mmol; organic solvent, 20 ml tetrahydrofuran; initial water activity, 0.63; temperature, 40 °C; enzyme dosage, 100 mg enzyme powder in this reaction system), the highest enzyme activity (3.71 ± 0.13 μmol/h/mg) could be obtained. The conversion could reach 91.42 ± 2.43% after 24 h and the regioselectivity of the CSL-catalyzed acylation was highly specific at the C-6′ position in the glucose moiety of arbutin (>99%). CSL has been proven to be an excellent candidate as a catalyst for highly regioselective acylation of arbutin.

Graphical abstractRegioselective acylation of arbutin catalyzed by CSL.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Arbutin; Regioselective acylation; Lipase from Candida sp.; Transesterification
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Highly efficient and regioselective acylation of arbutin catalyzed by lipase from Candida sp.
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 5, May 2015, Pages 789–792
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us