fulltext.study @t Gmail

Purification and characterization of a novel enzyme produced by Catenovulum sp. LP and its application in the pre-treatment to Ulva prolifera for bio-ethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
34338 45018 2015 8 PDF Available
Title
Purification and characterization of a novel enzyme produced by Catenovulum sp. LP and its application in the pre-treatment to Ulva prolifera for bio-ethanol production
Abstract

•A novel depolymerase to degrade PU was reported.•The depolymerase degraded PU efficiently, which also declined the viscosity of PU noticeably.•The enzymatic hydrolysis of PU was first reported.

The high viscosity of polysaccharides from Ulva prolifera (PU) is one reason that impeded the high production of bio-ethanol by U. prolifera. A depolymerase with high specificity was first isolated from Catenovulum sp. LP. The 75.5 kDa depolymerase was purified 42.35-fold to homogeneity with 22.86 U/mg specific activity. The enzyme showed the highest activity at the optimal conditions of pH 6.0 and 35 °C. It also showed high stability with a Half-life (t1/2) of 1386 min at 35 °C. It retained 100% of its residual activity in 10 mM EDTA, 1,10-phenanthrolin, or Tween 80 after incubation of 2 h. The depolymerase showed high efficiency to PU for reducing sugar production, which reached 50.2% yield in 6 h. However, the commercial enzymes showed no hydrolysis to PU. The viscosity of 1.2% PU noticeable declined from initially 1127 to 7.2 mPa s in 95 min of hydrolysis even in the room temperature. The application of depolymerase with these unique properties could bring promising prospects for enzymatic pretreatment of the bio-ethanol production from U. prolifera biomass.

Keywords
Depolymerase; Polysaccharides from Ulva prolifera; Purification; Characterization; Bio-ethanol
First Page Preview
Purification and characterization of a novel enzyme produced by Catenovulum sp. LP and its application in the pre-treatment to Ulva prolifera for bio-ethanol production
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 5, May 2015, Pages 799–806
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us