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Radial scale-down of packed bed chromatography in a thin cylindrical tube for preparative media

Paper ID Volume ID Publish Year Pages File Format Full-Text
34342 45018 2015 7 PDF Available
Title
Radial scale-down of packed bed chromatography in a thin cylindrical tube for preparative media
Abstract

•Chromatography columns were scaled down by reducing internal diameters.•Preparative agarose- and polymer-based gels were packed in thin microcolumns.•Packing quality, dynamic binding capacity and selectivity were compared.•Microcolumns showed comparable separation efficiency with lab-scale columns.

Microscale packed-bed chromatography columns (5 to 80 μL) were designed with cylindrical polyetheretherketone (PEEK) tubes for preparative ion exchange media by reducing internal column diameters with retained heights. Q Sepharose Fast Flow, soft agarose-based media, and POROS HQ, rigid polystyrene-based media, were packed in a thin column under the pressure of 0.3 MPa equally for various sizes of internal diameter ranging from 0.25 to 1 mm. The minimum internal diameter of tube was limited by the mean diameter of gel, thereby media could be packed in a plastic tube column that was no smaller than 5 times gel diameter, while maintaining comparable resin density. The microcolumn quantitatively showed equivalent performance when measured by total bound protein in breakthrough curve for both gels. Qualitative adsorption and desorption profiles showed that rigid gel exhibited slight increase in adsorption and separation efficiency as the column diameter decreased from 1 mm to 0.5 mm. The microscale PEEK tube column showed successful comparable performance with laboratory-scale liquid chromatography behavior, enabling it to offer a novel scale-down approach for the development of biopharmaceutical production process.

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Keywords
Microcolumn; Scale-down chromatography; Soft and rigid gels; Preparative media; Polyetheretherketone tubeAs, asymmetry factor; BSA, bovine serum albumin; CV, column volume; DBC, dynamic binding capacity; HETP, height equivalent theoretical plate; hIgG, hu
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Radial scale-down of packed bed chromatography in a thin cylindrical tube for preparative media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 5, May 2015, Pages 839–845
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us