fulltext.study @t Gmail

In vitro antiviral activities of enzymatic hydrolysates extracted from byproducts of the Atlantic holothurian Cucumaria frondosa

Paper ID Volume ID Publish Year Pages File Format Full-Text
34346 45018 2015 9 PDF Available
Title
In vitro antiviral activities of enzymatic hydrolysates extracted from byproducts of the Atlantic holothurian Cucumaria frondosa
Abstract

•Bioactive compounds were extracted from aquapharyngeal bulb of Cucumaria frondosa.•Enzymatic extraction permitted the recovery of antiherpetic compounds on our model.•Antiherpetic activities were highlighted on enzymatic hydrolysates of aquapharyngeal bulb.•High molecular weight molecules were demonstrated as active against HSV-1.

Herpes Simplex virus 1 (HSV-1), responsible for the common cold sore, can also lead to serious infections in immunocompromised people. Current antiviral chemotherapies face obstacles including the toxicity of therapeutic molecules, interference with normal cellular metabolism, genetic variability and the incurable nature of latent infections. Therefore, the search for new treatments is a public health issue. Marine invertebrates have held great potential for finding novel antiviral compounds. Little is known, about the antiviral activities of compounds isolated from holothurians. In New Brunswick, holothurian is fished for its edible bodywall and muscle, but its processing generates high amounts of byproducts. In vitro evaluation of the anti-HSV-1 activity by cell viability was performed on nine hydrolysates obtained by enzyme-assisted extraction and four solvent extractions from aquapharyngeal bulb and internal organs of Cucumaria frondosa at an MOI of 0.001 ID50/cells. After 72 h, four enzymatic hydrolysates from the aquapharyngeal bulb presented effective antiherpetic activities (EC50 = 7.2–15.2 μg/mL). After evaluation at a higher MOI (0.01 ID50/cells), the most efficient extract (Papain hydrolysate) was fractionated to identify the active fraction. The fraction superior to 100 kDa showed the highest antiherpetic activity (EC50: 18.2 μg/mL). In conclusion, upgrading byproducts of sea cucumber fisheries offers new sources of bioactive molecules.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Cucumaria frondosa; Enzymatic hydrolysis; Herpes simplex virus type 1; Byproducts
First Page Preview
In vitro antiviral activities of enzymatic hydrolysates extracted from byproducts of the Atlantic holothurian Cucumaria frondosa
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 5, May 2015, Pages 867–875
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us