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Enzymatic synthesis of gold nanoparticles using sulfite reductase purified from Escherichia coli: A green eco-friendly approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
34354 45019 2015 10 PDF Available
Title
Enzymatic synthesis of gold nanoparticles using sulfite reductase purified from Escherichia coli: A green eco-friendly approach
Abstract

•A sulfite reductase (MW; 116 kDa) was purified to homogeneity from Escherichia coli.•Purified enzyme was successfully used for synthesis of gold nanoparticles.•Gold nanoparticles showed strong antifungal activity against human pathogenic fungi.•Gold nanoparticles had no obvious cytotoxicity for Vero and Hep-2 cell lines in vitro.•Bioactive non-toxic gold nanoparticles have potential to use in biology and medicine.

Biological synthesis of nanomaterials using microorganisms is a growing field of industrial microbiology due to the numerous beneficial properties while lower toxicity for biological systems. We have developed a cell-free viable approach for synthesis of gold nanoparticles using α-NADPH-dependent sulfite reductase purified from Escherichia coli. The enzyme was purified by ion exchange chromatography on DEAE Sephadex A-50 and its molecular weight was determined by gel filtration on Sephacryl S-300. The purified enzyme with molecular weight of 116 kDa composed of two subunits of 75 and 41 kDa was successfully used for cell-free synthesis of gold nanoparticles which were spherical with an average size of 10 nm and a zeta potential of −30 ± 0.2. Gold nanoparticles showed strong antifungal activity toward a wide range of human pathogenic fungi. Evaluation of the in vitro cytotoxicity of gold nanoparticles showed no toxicity for two cell lines, i.e., Vero and Hep-2 at the concentrations ranged from 0.31 to 10%. Taken together, biologically active non-toxic gold nanoparticles green synthesized by a sulfite reductase of E. coli in the present work are potential candidates for application in different disciplines of medicine and biology from diagnostics to drug delivery systems, combating infectious diseases and anticancer therapies.

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Keywords
Gold nanoparticles; Green chemistry; Sulfite reductase; Cytotoxicity; Escherichia coli; Antifungal activity
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Enzymatic synthesis of gold nanoparticles using sulfite reductase purified from Escherichia coli: A green eco-friendly approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 7, July 2015, Pages 1076–1085
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us