fulltext.study @t Gmail

Performance evaluation of a proliferation chamber with external stirred conditioning tank for expansion of a suspendable stem cell model

Paper ID Volume ID Publish Year Pages File Format Full-Text
34359 45019 2015 9 PDF Available
Title
Performance evaluation of a proliferation chamber with external stirred conditioning tank for expansion of a suspendable stem cell model
Abstract

•U937 cells were cultured in a proliferation chamber with external conditioning tank.•The bioreactor reduced shear stress exerted on the cells as a suspendable cell model.•U937 cells showed considerable cell proliferation in the culture system.•Original U937 cells were round-shaped cells with a roundness of more than 0.9.•U937 cells maintained their original phenotypes fairly when cultured in the system.

Performance of a proliferation chamber with external stirred conditioning tank (PC-ESCT) was evaluated to expand a cell line as a model of suspendable stem cells. A hematopoietic cell line (U937) showed considerable cell proliferation in the PC-ESCT at seeding densities of 104 and 105 cells/mL (97.5 ± 1.7 and 24.5 ± 1.9-fold), as compared to the cultures in T-flask (80 ± 2.8 and 21.5 ± 1.4-fold) and stirred tank bioreactor (STB) (no detectable increase and 2.85 ± 0.8-fold) after 8 days of culture. The round-shaped cells appeared dominantly in the T-flask and PC-ESCT cultures, while few cells could maintain their original phenotype or survive in the STB system during the culture period. The cell surface marker analysis of CD14 for testing self-renewing proliferation of the cells also coincide with the cell appearance and quantitative morphological analysis based on the roundness parameter. The present study showed the potential of the PC-ESCT for the expansion of suspendable stem cells in vitro.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Proliferation chamber; External stirred conditioning tank; Suspendable stem cell; Shear stress; Cell roundnessPC-ESCT, proliferation chamber with external stirred conditioning tank; STB, stirred tank bioreactor; HSCs, hematopoietic stem cells; DO, dissolv
First Page Preview
Performance evaluation of a proliferation chamber with external stirred conditioning tank for expansion of a suspendable stem cell model
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 7, July 2015, Pages 1110–1118
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us