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Guava (Psidium guajava Linn.) leaf extract promotes glucose uptake and glycogen accumulation by modulating the insulin signaling pathway in high-glucose-induced insulin-resistant mouse FL83B cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
34361 45019 2015 8 PDF Available
Title
Guava (Psidium guajava Linn.) leaf extract promotes glucose uptake and glycogen accumulation by modulating the insulin signaling pathway in high-glucose-induced insulin-resistant mouse FL83B cells
Abstract

•We reveal the hypoglycemic mechanisms of GvEx in high-glucose-induced insulin-resistant liver cells.•Insulin-resistant status was improved by GvEx through modulating the insulin signaling cascade.•GvEx increases glucose uptake and enhanced glycogen accumulation.

The effects of aqueous guava leaf extract (GvEx) on insulin resistance were evaluated in high glucose-induced insulin-resistant mouse FL83B cells. Glucose uptake and glycogen content were determined and western blot analysis was performed to study the anti-diabetic effects of GvEx. Our results showed that administration of 200 and 400 μg/mL GvEx resulted in a significant increase in glucose uptake in both normal and insulin-resistant cells. Glycogen content in cells was significantly higher after incubation with GvEx (200, 400 μg/mL) than that in control group (p < 0.05). Further, western blot analysis revealed that 200 and 400 μg/mL GvEx significantly enhanced the levels of insulin-related signaling components, including insulin receptor (IR), as well as enhancing the phosphorylation of the insulin receptor (p-IR (Tyr)), insulin receptor substrate (p-IRS (Tyr)), p85 regulatory subunit of phospho-inositide 3 kinase (PI3K (p85)), protein kinase B (p-Akt (Ser)), glucose transporter 2 (Glut 2), and total glycogen synthase. In conclusion, GvEx improved insulin resistance by promoting glucose uptake and enhancing glycogen content via modulation of the insulin signaling pathway in high glucose-induced insulin-resistant mouse FL83B cells.

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Keywords
Glucose uptake; Glycogen accumulation; Insulin resistance; Psidium guajava leaf
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Guava (Psidium guajava Linn.) leaf extract promotes glucose uptake and glycogen accumulation by modulating the insulin signaling pathway in high-glucose-induced insulin-resistant mouse FL83B cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 7, July 2015, Pages 1128–1135
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us