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Fatty acids and global metabolites profiling of Dunaliella tertiolecta by shifting culture conditions to nitrate deficiency and high light at different growth phases

Paper ID Volume ID Publish Year Pages File Format Full-Text
34377 45020 2014 9 PDF Available
Title
Fatty acids and global metabolites profiling of Dunaliella tertiolecta by shifting culture conditions to nitrate deficiency and high light at different growth phases
Abstract

•Nitrate-deficient and high light induced saturation of fatty acids in D. tertiolecta.•Relative levels of metabolites acting as osmolytes were decreased under stress.•Altering conditions during exponential phase led significant changes in fatty acids.•Shifting culture condition at exponential phase was suggested for biomass production.

In this study, Dunaliella tertiolecta cells were shifted to nitrate-deficient (ND) and/or high light (HL) conditions at different growth phases. Profiling of global metabolites and fatty acids of D. tertiolecta cells were performed by shifting cultivation condition at exponential (EX) and stationary (ST) phases. ND and/or HL induced saturation of fatty acids by increasing saturated fatty acid and monounsaturated fatty acid and decreasing polyunsaturated fatty acid in D. tertiolecta cultures. The relative levels of metabolites like proline, valine, isoleucine, and myoinositol, which act as osmolytes, and ergosterol and glycerol, which are related to the osmoregulatory mechanism, decreased to a greater extent by ND than HL shifts. In addition, the effects of these shifts at the EX phase were observed to be more significant in most of the fatty acids. The results of this research might be applicable to the interpretation of metabolic synthesis and fatty acid production, and to the optimization of cultivation conditions for the use of D. tertiolecta and other microalgae as biofuels or nutraceutical sources.

Keywords
Dunaliella tertiolecta; Metabolic profiling; High light; Nitrate deficiency; Culture condition shift
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Fatty acids and global metabolites profiling of Dunaliella tertiolecta by shifting culture conditions to nitrate deficiency and high light at different growth phases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 6, June 2014, Pages 996–1004
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us