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Negative chromatography: Progress, applications and future perspectives

Paper ID Volume ID Publish Year Pages File Format Full-Text
34378 45020 2014 7 PDF Available
Title
Negative chromatography: Progress, applications and future perspectives
Abstract

•Reviews the current progress of negative chromatography (NC) and its application.•Product flows through the negative chromatographic column.•The performance of NC is independent of adsorbent binding capacity.•NC appears as an alternative to conventional chromatography.•NC has been successfully applied to purify various therapeutic proteins.

In negative chromatography, the impurities bind on the adsorbent, and the product is allowed to flow through the chromatographic column. Negative chromatography is an alternative to positive chromatography under certain circumstances and has been used to purify various biomolecules. For this review, a detailed survey of the performance of reported studies on negative chromatography was conducted. The applications of negative chromatography in the capture and intermediate purification steps for biomolecules (e.g., plasmid DNA, antibodies, enzymes, hemoglobin, virus particles and cells) are reviewed. The negative chromatographic adsorbents adsorb the impurities through surface charge, hydrophobic interaction at specific sites on the surface, hydrophobic interaction, hydrogen bonding and functional groups. Examples of applications of negative chromatography according to the type of chromatography matrix used are summarized and discussed. In addition, the effects of operating conditions (initial protein concentration, buffer ions, pH and salt concentration) are discussed, and the criteria for choosing negative or positive chromatography are summarized. The literature survey showed that there will be future limitations and challenges ahead in implementation of negative chromatography. Possible solutions to the limitations and challenges of negative chromatography and future trends for developing negative chromatography are discussed.

Keywords
Negative chromatography; Flow-through chromatography; Protein purification; Adsorbent; Recombinant protein; Downstream bottleneck
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Negative chromatography: Progress, applications and future perspectives
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 6, June 2014, Pages 1005–1011
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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