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Isolation and identification of an anticancer compound from the bark of Acer tegmentosum Maxim

Paper ID Volume ID Publish Year Pages File Format Full-Text
34381 45020 2014 8 PDF Available
Title
Isolation and identification of an anticancer compound from the bark of Acer tegmentosum Maxim
Abstract

Highlight•A compound isolated from the butanol soluble fraction of ATM extract by RP-HPLC.•Compound was isolated from the ATM extracts for the first time.•The isolated compound was identified to be salidroside.•Salidroside effects were evaluated on HepG2 cells.•Results show that salidroside, exhibits high apoptosis activity in HepG2 cells.

In this study, we investigated the anticancer activity of a newly isolated compound from Acer tegmentosum Maxim (ATM) in HepG2 cells. This compound was isolated by reverse-phase high-performance liquid chromatography (RP-HPLC) in a butanol-soluble fraction, which was shown to have the strongest anticancer activity. The isolated compound was identified as salidroside using multiple nuclear magnetic resonance (NMR) techniques, including 1H, 13C, correlated spectroscopy (COSY), heteronuclear single quantum coherence (HSQC), and heteronuclear multiple bond correlation (HMBC), as well as electrospray ionization mass spectroscopy (ESI/MS). The activity of salidroside was evaluated in HepG2 cells by analyzing cell proliferation, cell cycle distribution, Hoechst 33342 staining, and Western blots of apoptotic regulatory proteins. The results show that salidroside, an anticancer compound from ATM, exhibits strong apoptotic activity in HepG2 cells. Therefore, ATM extracts could be used as chemotherapeutic agent to induce apoptosis in hepatoblastoma cells.

Keywords
Acer tegmentosum Maxim; Anticancer; Salidroside; HepG2 cells
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Isolation and identification of an anticancer compound from the bark of Acer tegmentosum Maxim
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 6, June 2014, Pages 1032–1039
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us