fulltext.study @t Gmail

Bioengineering yeast to enhance folate levels in wine

Paper ID Volume ID Publish Year Pages File Format Full-Text
34392 45023 2015 6 PDF Available
Title
Bioengineering yeast to enhance folate levels in wine
Abstract

•Folate (vitamin B9) levels vary widely in commercial wines.•The folate level in wine is generated by yeast during fermentation.•Folate levels in wine can be boosted by metabolic engineering of yeast.

Fortification of food systems using modern biotechnology represents an exciting approach to improve health in different human sectors that require an increased dietary intake of specific nutrients [1]. One of these nutrients, folate (vitamin B9), is an important intermediate in cellular metabolism. Its deficiency has been linked to severe health problems, both in infants and adults [2]. Therefore, we examined a relevant food system, wine fermentation, for folate. We measured folate in commercial wines, and found variable levels of this vitamin. Lab-scale fermentations revealed that folate levels were determined primarily by the yeast used to ferment the wine, with a dependence on the particular strain used. We also undertook the bioengineering of yeast to determine if folate concentration in wine could be increased. Using molecular techniques, overexpression of two folate biosynthesis genes in wine yeast was undertaken. Overexpression of FOL2, but not ABZ1, resulted in elevated folate levels in wine. Our results demonstrate the utility of bioengineering yeast for the vitamin fortification of wine.

Keywords
Folate; Wine; Vitamin; Biofortification
First Page Preview
Bioengineering yeast to enhance folate levels in wine
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 2, February 2015, Pages 205–210
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us