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The multi-chitinolytic enzyme system of the compost-dwelling thermophilic fungus Thermomyces lanuginosus

Paper ID Volume ID Publish Year Pages File Format Full-Text
34396 45023 2015 8 PDF Available
Title
The multi-chitinolytic enzyme system of the compost-dwelling thermophilic fungus Thermomyces lanuginosus
Abstract

•Novel family 18 chitinases were identified in T. lanuginosus SSBP.•Presence of a multi-chitinolytic enzyme system was confirmed in this fungus.•Chit1 and Chit2 have both exo- and endochitinase activities.•Chit1 is efficient for biodegradation of chitinous waste.•Chit2 showed antifungal activity against P. verrucosum and A. niger.

The recent sequencing of the Thermomyces lanuginosus SSBP genome by our group has revealed four putative family 18 chitinases. In this study, three novel chitinase genes (chit2, chit3 and chit4) and the previously-reported chit1 gene were cloned from T. lanuginosus SSBP. chit1, encoding a 44.1 kDa protein, and chit2, encoding a 36.6 kDa protein, were successfully expressed in Pichia pastoris. The recombinant Chit1 and Chit2 enzymes exhibited optimum activity at pH 5.0 and pH 4.0, respectively. Chit1 had optimal activity at 50 °C and retained 56% of its activity at 60 °C after 30 min, while Chit2 was optimally active at 40 °C and retained 71% of its activity at 50 °C after 60 min. Both enzymes produced chitobiose as the major product using different substrates. Chit2 displayed antifungal activity against Penicillium verrucosum and Aspergillus niger. These activities could be useful in the environmental degradation of chitinous wastes as well as for biotechnological applications.

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Keywords
Chitin; Chito-oligosaccharides; Thermomyces lanuginosus SSBP; Exo/endochitinases; Crude shrimp shell powder
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The multi-chitinolytic enzyme system of the compost-dwelling thermophilic fungus Thermomyces lanuginosus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 2, February 2015, Pages 237–244
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us