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Development of a simultaneous extraction and acid hydrolysis process for recovery of paclitaxel from plant cell cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
34401 45023 2015 6 PDF Available
Title
Development of a simultaneous extraction and acid hydrolysis process for recovery of paclitaxel from plant cell cultures
Abstract

•MAE/acid hydrolysis simultaneous process was developed.•Acid hydrolysis broke down a glycosidic bond of glycoside.•The paclitaxel yield was increased by the cleavage of glycoside.•The extraction time was reduced by simultaneous process.

In this study, a microwave-assisted extraction (MAE)/acid hydrolysis simultaneous process was developed for recovering the anticancer agent paclitaxel from plant cell cultures of Taxus chinensis. The optimal pH of the extraction solution (90% aqueous methanol) for hydrolysis was 2.2 at fixed extraction time (6 min), ratio of extraction solution to biomass (1:1, v/w), and extraction temperature (40 °C). In the MAE/acid hydrolysis simultaneous process, the paclitaxel recovery was 2.2-fold higher than in the existing extraction methods. Regarding changes in the content of glycoside (7-xylosyl paclitaxel as sugar-binding paclitaxel) and paclitaxel depending on the inclusion of acid hydrolysis in the MAE process, the content of 7-xylosyl paclitaxel decreased after acid hydrolysis whereas the content of paclitaxel increased. Based on this result, it was confirmed that acid hydrolysis breaks down a glycosidic bond of glycoside (sugar-binding paclitaxel), and so the recovery of paclitaxel increases.

Keywords
Plant cell culture; Paclitaxel; Microwave-assisted extraction (MAE); Acid hydrolysis; Simultaneous process
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Development of a simultaneous extraction and acid hydrolysis process for recovery of paclitaxel from plant cell cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 2, February 2015, Pages 279–284
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us