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Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
34402 45023 2015 9 PDF Available
Title
Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli
Abstract

•High-level production of MBP-CCR3 in E. coli has been obtained.•Fos choline-14 is a good surfactant for the solubilization and stabilization of MBP-CCR3.•MBP-CCR3 can be conveniently and efficiently purified with chromatography.•MBP-CCR3 expressed in E. coli has high binding affinity with its endogenous ligands.

Human C-C chemokine receptor 3 (CCR3), belonging to the G protein-coupled receptor (GPCR) family, plays a central role in various allergic diseases. Although good understanding on this protein is of critical importance to drug design, studies on the structure and functions of CCR3 are still limited, mainly due to the difficulties to obtain a large quantity of pure and functional protein. Here we report high-level production of human CCR3 fused to maltose binding protein (MBP) in Escherichia coli. Over-expression of MBP-CCR3 was achieved by systematically optimizing expression conditions, and Fos choline-14 was found to be an optimum surfactant for solubilization and stabilization of MBP-CCR3 in purification. After the two-step chromatography purification, the yield of MBP-CCR3 was 2–4 mg/l culture. The binding activity of purified MBP-CCR3 was verified by binding with its endogenous ligands CCL11 and CCL24, with KD of 2.90 × 10−6 and 1.81 × 10−6 M, respectively. This is the first report for that MBP fused recombinant CCR3 (MBP-CCR3) produced in E. coli still maintains its binding activity with its endogenous ligands, which provides an efficient way to produce functional GPCR proteins at large scale for further structural and functional studies.

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Keywords
G protein-coupled receptors; CC chemokine receptor 3; Maltose binding protein; Escherichia coli; Heterologous expression
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Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 2, February 2015, Pages 285–293
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us