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A Kunitz-type serine protease inhibitor from Butea monosperma seed and its influence on developmental physiology of Helicoverpa armigera

Paper ID Volume ID Publish Year Pages File Format Full-Text
34405 45023 2015 6 PDF Available
Title
A Kunitz-type serine protease inhibitor from Butea monosperma seed and its influence on developmental physiology of Helicoverpa armigera
Abstract

•Butea monosperma protease inhibitor (BmPI) on 2D resolved as ∼14 kDa protein.•Multiple Sequence Analysis data indicated a Kunitz-type inhibitor.•BmPI exhibited a broad range of pH (4–10) and temperature (10–80 °C) activity.•Kinetic studies revealed BmPI as a competitive inhibitor.•BmPI retarded growth of Helicoverpa armigera larvae and affected fecundity and fertility.

A protease inhibitor from the seeds of Butea monosperma (BmPI) was purified, characterized and studied for its influence on developmental physiology of Helicoverpa armigera. BmPI on two-dimensional separations indicated the presence of a 14 kDa protein with an isoelectric point in the acidic region (pI 5.6). Multiple Sequence Analysis data suggested that the BmPI contains a sequence motif which is conserved in various trypsin and chymotrypsin inhibitors of Kunitz-type. The inhibitor exhibited trypsin inhibitory activity in a broad range of pH (4–10) and temperature (10–80 °C). The enzyme kinetic studies revealed BmPI as a competitive inhibitor with a Ki value of 1.2 × 10−9 M.In vitro studies with BmPI indicated measurable inhibitory activity on total gut proteolytic enzymes of H. armigera (IC50 2.0 μg/ml) and bovine trypsin. BmPI supplemented artificial diet caused dose dependent mortality and reduction in growth and weight. The fertility and fecundity of H. armigera, declined whereas the larval–pupal duration of the insect life cycle extended. These detrimental effects on H. armigera suggest the usefulness of BmPI in insect pest management of food crops.

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Keywords
Helicoverpa armigera gut proteases; Trypsin inhibitor; Kinetics; Stability; BLAST; Kunitz-type inhibitor
First Page Preview
A Kunitz-type serine protease inhibitor from Butea monosperma seed and its influence on developmental physiology of Helicoverpa armigera
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 2, February 2015, Pages 311–316
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering