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Soft-sensor assisted dynamic investigation of mixed feed bioprocesses

Paper ID Volume ID Publish Year Pages File Format Full-Text
34411 45025 2013 9 PDF Available
Title
Soft-sensor assisted dynamic investigation of mixed feed bioprocesses
Abstract

•We present a dynamic method for the physiological investigation of mixed feed bioprocesses.•A combination of pulse/ramp dynamics, infrared spectroscopy (FTIR) and soft sensors was used.•Residual l-arabinose concentrations were measured in-line (FTIR) and used for process control.•Physiological capabilities of a E. coli C41 mixed feed pBAD expression system were established.•The method is generically applicable for the investigation of mixed feed bioprocesses.

Recombinant mixed feed bioprocesses are characterized by the controlled feeding of multiple defined carbon sources aiming at increased productivities. However, mixed feed process design is challenging due to physiological constraints such as adaptation times and catabolite repression.A novel soft-sensor assisted dynamic method that allows the science-based process design with respect to co-utilization of primary and secondary substrate was developed. The method is based on the control of the specific uptake rates of primary and secondary substrate via a combination of a rate-based soft sensor and in-line infrared spectroscopy. Maximum secondary substrate specific uptake rates and adaptation times are determined by a combination of dynamic pulse and ramp experimentation.The power of the presented method was demonstrated on a recombinant Escherichia coli pBAD mixed feed process with d-glucose as primary and l-arabinose as secondary carbon source. Onset of catabolite repression was observed once a total specific substrate uptake rate of 1.0 g/gh was exceeded. Adaptation times to l-arabinose were determined as ∼10 min.The presented method can be considered generically applicable for the physiological investigation of mixed feed systems. Furthermore, metabolic capabilities of the promising but yet unexplored E. coli pBAD mixed feed system were explored for the first time.

Keywords
Bioprocess technology; Mixed feed; Dynamic experiments; Physiological process control; Soft-sensors; In-line Fourier transformation infrared spectroscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 12, December 2013, Pages 1839–1847
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us