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Biodegradation of feather keratin with a PEGylated protease of Chryseobacterium gleum

Paper ID Volume ID Publish Year Pages File Format Full-Text
34427 45025 1963 12 PDF Available
Title
Biodegradation of feather keratin with a PEGylated protease of Chryseobacterium gleum
Abstract

•Keratinolytic protease of C. gleum was covalently modified by attachment of polyethylene glycol.•Modified protease activity was remarkably increased by PEGylation.•It showed non-immunogenicity with improved pH, thermal, storage and solvent stability.•Modified protease effectively demonstrated its reusability in keratin degradation.

Present study deals with the covalent modification of keratinolytic protease of Chryseobacterium gleum with higher enzyme activity, improved stability, non-immunogenicity and reusability. Protease of C. gleum showing feather degradation ability was modified by covalent attachment to polyethylene glycol. This modification culminated the change in electrophoretic mobility of protease in acrylamide gel. The modified enzyme showed 1.4 times more catalytic activity with better stability than native in aqueous system containing whole feathers as keratin. It showed improved pH, thermal, storage and solvent stability with a broadened range of pH (7–9) and temperature (25–50 °C) than native. The differentiation between modified and native enzyme was authenticated through UV–vis spectroscopy, SEM, XRD, FTIR and DSC. This modification of protease proved to be non-immunogenic in rats. The enzyme extracted after first run could be used for several cycles which clearly demonstrated its reusability in catalytic bioprocess of keratin degradation.

Keywords
Keratinolytic protease; Polyethylene glycol; Covalent modification; Improved stability; Non-immunogenic protein; Reusability
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Biodegradation of feather keratin with a PEGylated protease of Chryseobacterium gleum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 12, December 2013, Pages 1952–1963
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us