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Unveiling the potential of metal-tolerant fungi for efficient enzyme production

Paper ID Volume ID Publish Year Pages File Format Full-Text
34443 45026 2014 9 PDF Available
Title
Unveiling the potential of metal-tolerant fungi for efficient enzyme production
Abstract

The present study first time reports the utilization of metal tolerant fungi as a potential source for efficient enzyme production. The purification and characterization of alkaline protease from an indigenous zinc-metal tolerant fungal isolate Aspergillus flavus NJP08 has been demonstrated. The specific activity of enzyme was determined as 89.1 U mg−1 which is found to be the highest among the reported Aspergillus flavus isolates so far. The protease was purified 55.87 fold up to homogeneity and identified as “alkaline protease” with a molecular mass of 33 kDa. The N-terminal sequence was GLTTQKSAPWGLG which showed high similarity with other reported proteases of genus Aspergillus. Physico-chemical characterization of enzyme revealed an estimated half-life of >20 h with aliphatic and GRAVY index values of 79.65 and −0.161 respectively, depicting high thermo-stability and secretory nature of protease. The protease was active within the temperature range of 25–50 °C with an optimum temperature of 50 °C and was stable in the pH range of 6.0–11.0. The enzyme was activated by Ca2+ and Fe2+ ions, partially inhibited by Cu2+ ions and strongly inhibited by PMSF. High enzyme stability in presence of various detergents further strengthens enzyme applicability in industrial applications.

Keywords
BCA, bicinchoninic acid; CBB, coomassie brilliant blue; DEAE cellulose, diethylaminoethyl cellulose; EDTA, ethylene diamine tetraacetic acid; GRAVY, grand average of hydropathy; MALDI–TOF-MS, matrix-assisted laser desorption/ionization time of flight mass
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Unveiling the potential of metal-tolerant fungi for efficient enzyme production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 11, November 2014, Pages 1858–1866
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us