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Inulinase production by the yeast Kluyveromyces marxianus with the disrupted MIG1 gene and the over-expressed inulinase gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
34444 45026 2014 8 PDF Available
Title
Inulinase production by the yeast Kluyveromyces marxianus with the disrupted MIG1 gene and the over-expressed inulinase gene
Abstract

•The glucose-derepressed mutant produced 94.6 units of inulinase activity per ml.•The native inulinase gene was over-expressed in the glucose-derepressed mutant.•The recombinant yeast strain KM-526 obtained yielded 133.5 units of inulinase activity per ml.

Kluyveromyces marxianus has been widely used in food industries and inulinase produced by the yeast has many applications. In order to greatly enhance inulinase production by the yeast, it is very important to both derepress glucose repression and overexpress the native inulinase gene. The MIG1 gene in the yeast K. marxianus KM-0 was disrupted. It was found that a glucose-derepressed mutant (KM-69) of K. marxianus KM-0 could produce 94.6 units of inulinase activity per ml in the inulin medium within 36 h. In order to further increase inulinase production, the native inulinase gene was over-expressed in the glucose-derepressed mutant. During the 10-l fermentation, the recombinant strain KM-526 yielded 133.5 units of inulinase activity per ml within very short time (24 h). The results also showed that inulinase produced by the recombinant strain KM-526 could more effectively convert inulin into monosaccharides than that produced by K. marxianus KM-0. The results indicate that it is very important to delete the MIG1 gene in K. marxianus KM-0 and over-express the native INU1 gene in order to further enhance inulinase yield within the short time by this yeast and for efficient hydrolysis of inulin. The recombinant strain KM-526 and its inulinase have highly potential applications in food industries.

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Keywords
Kluyveromyces marxianus; Inulinase overproduction; Glucose repression; Over-expression; Fermentation
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Inulinase production by the yeast Kluyveromyces marxianus with the disrupted MIG1 gene and the over-expressed inulinase gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 11, November 2014, Pages 1867–1874
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us