fulltext.study @t Gmail

Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens

Paper ID Volume ID Publish Year Pages File Format Full-Text
34452 45026 2014 7 PDF Available
Title
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
Abstract

•Rotavirus VLPs carrying large sequences in the inner space were produced.•Chimeric VLPs seem to be specifically endocyted by murine dendritic cells.•Mice were vaccinated with chimeric VLPs.•In vivo cytotoxic assays were used to evaluate cellular immune response.•A strong cytotoxic immune response was elicited against the heterologous sequence.

The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
CTL responses; Heterologous antigens; Rotavirus-derived DLPs as carrier; Vector vaccine; Baculovirus system
First Page Preview
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 11, November 2014, Pages 1929–1935
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us