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A novel strategy for sequential co-culture of Clostridium thermocellum and Clostridium beijerinckii to produce solvents from alkali extracted corn cobs

Paper ID Volume ID Publish Year Pages File Format Full-Text
34454 45026 2014 9 PDF Available
Title
A novel strategy for sequential co-culture of Clostridium thermocellum and Clostridium beijerinckii to produce solvents from alkali extracted corn cobs
Abstract

•A new strategy for co-culture of C. thermocellum and C. beijerinckii was proposed.•Important parameters for the co-culture were investigated and optimized.•The co-culture produced 19.9 g L−1 of solvents from 88.9 g L−1 of AECC in 200 h.

Increasing concern on fossil fuels depletion and global environmental deterioration has recently renewed interest in butanol production from lignocellulose by acetone–butanol–ethanol (ABE) fermentation. Unfortunately, there was barely microorganism possessing the compound ability of lignocellulose decomposition and solvents production natively. Comparing with complicated genetic and metabolic engineering strategy to engineer native cellulolytic or solventogenic bacteria, mixed culture is a convenient and feasible approach for ABE fermentation by consolidated bioprocessing (CBP) from lignocellulose. But few communities of microorganisms succeeded in producing butanol of high titer or productivity without adding butyrate. Here a novel strategy for sequential co-culture of Clostridium thermocellum ATCC 27405 and Clostridium beijerinckii NCIMB 8052 was proposed to produce solvents efficiently in one pot reaction with alkali extracted corn cobs (AECC), a low-cost renewable feedstock, as the sole carbon source. In this strategy, soluble sugars accumulation by C. thermocellum hydrolyzing AECC was considered to be paramount for the CBP and was promoted considerably by contrast with previous co-culture studies. Under the combinatorial optimal culture parameters for sugars accumulation and ABE production, the CBP decomposed 88.9 g L−1 of AECC and manufactured ABE 19.9 g L−1 (acetone 3.96, butanol 10.9 and ethanol 5.04 g L−1) in 200 h without feeding butyrate.

Keywords
AEC, Calkali extracted deshelled corn cobs; ABE, acetone–butanol–ethanol; CBP, consolidated bioprocessing; CCR, carbon catabolite repression; YE, yeast extractABE fermentation; Consolidated bioprocessing; Sequential co-culture; Lignocellulose; Sugars accu
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A novel strategy for sequential co-culture of Clostridium thermocellum and Clostridium beijerinckii to produce solvents from alkali extracted corn cobs
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 11, November 2014, Pages 1941–1949
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us