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The hydrophobicity of the support in solid state culture affected the production of hydrophobins from Lecanicillium lecanii

Paper ID Volume ID Publish Year Pages File Format Full-Text
34467 45027 2015 6 PDF Available
Title
The hydrophobicity of the support in solid state culture affected the production of hydrophobins from Lecanicillium lecanii
Abstract

•We produced hydrophobins from Lecanicillium lecanii in solid substrate culture.•Hydrophobicity of supports of solid substrate culture influenced surface activities of classes I and II HfbL.•Purity of chitin as substrate increase the HfbL production.•Class I HfbL only was produced on hydrophobic support and presented the highest surface activity.•Class II HfbLs were produced on hydrophobic and hydrophilic supports.

Lecanicillium lecanii has been successfully employed to produce hydrophobin-like proteins (HfbL) in solid state and submerged cultures varying the type of solid inert support. This study shows the results on the effect of hydrophilic Perlite and hydrophobic Polyurethane inert supports in solid state cultures for production of HfbLs by L. lecanii. The hydrophobicity of the support employed in solid state cultures influenced the yields and surface activities of the class I and class II HfbLs produced by L. lecanii. Class I HfbL was only produced using the hydrophobic polyurethane foam support, showing high surface activity that reduced ca. 50% hydrophobicity of Teflon, whereas class II HfbLs were produced on both polyurethane foam and perlite supports, and they reduced ca. 50% the surface tension of water and ca. 25% reduction of the hydrophobicity of Teflon.

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Keywords
Hydrophobins; Chitin; Lecanicillium lecanii; Solid state culture; Polyurethane foam; Perlite
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The hydrophobicity of the support in solid state culture affected the production of hydrophobins from Lecanicillium lecanii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 1, January 2015, Pages 14–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us