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An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches

Paper ID Volume ID Publish Year Pages File Format Full-Text
34492 45030 2014 10 PDF Available
Title
An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches
Abstract

•OMICS study integrating siRNA application for ALDH1 silencing in keratinocytes.•The selected genes associated with skin barrier function and cytokines/chemokines regulated by ALDH1 downregulation.•ALDH1 function in skin keratinocytes and the further speculation for the genes of interest.•ALDH1 downregulation and its induced skin disease, i.e., atopic dermatitis.

The role of acetaldehyde dehydrogenase 1 (ALDH1) has been probed in several diseases, especially in various types of cancers. However, the role of ALDH1 in skin cells has not been well elucidated. In this study, we aimed to identify critical factors associated with ALDH1 in keratinocytes through gene and protein expression profiling approaches. To this end, we conducted serial OMICS studies, including DNA microarray analysis, integrated antibody–chip arrays, and the implementation of bioinformatics algorithms designed to integrate siRNA data upon ALDH1 silencing. Cumulatively, these approaches identified several novel genes and proteins in keratinocytes associated with the downregulation of ALDH1. These novel genes and proteins included CYP1A1, a member of the cytochrome family of enzymes; extracellular matrix genes; and cytokines and chemokines, which are believed to play important roles in ALDH1-associated skin diseases such as atopic dermatitis. By integrating the datasets obtained from these complementary high-throughput OMICS studies and utilizing the strengths of each method, we obtained new insights into the functional role of ALDH1 in skin cells. The approach used here could help contribute to our clinical understanding of ALDH1-associated diseases, and may also be broadly applicable to a wider range of diseases.

Keywords
ALDH1, acetaldehyde dehydrogenase 1; siRNA, small interfering RNA; PPI, protein–protein interactionsALDH1; siRNA; OMICS; Microarray; Keratinocytes
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An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 10, October 2014, Pages 1612–1621
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us