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Unwrapping the hydrolytic system of the phytopathogenic fungus Phoma exigua by secretome analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
34494 45030 2014 7 PDF Available
Title
Unwrapping the hydrolytic system of the phytopathogenic fungus Phoma exigua by secretome analysis
Abstract

•The secretome of phytopathogenic fungus P. exigua was deciphered for the first time.•Acetone proves to be best organic solvent for precipitation of Phoma secretome.•LC–MS/MS and zymography analysis revealed the presence of diverse GH family proteins with multicomponent endoglucanase.

The present work describes the secretome profiling of a phytopathogenic fungus, Phoma exigua by liquid chromatography coupled tandem mass spectrometry (LC–MS/MS) based proteomics approach to highlight the suites of enzymes responsible for biomass hydrolysis. Mass spectrometry identified 33 proteins in the Phoma secretome when grown on α-cellulose as the sole carbon source. The functional classification revealed a unique extracellular enzyme system mainly belonging to the family of glycosyl hydrolase proteins (52%). This hydrolytic system consisted of cellulases (endo-1,4-β-glucanase, cellobiohydrolase I, exoglucanase, and β-glucosidase), hemicellulases (1,4-β-xylosidase and endo-1,4-β-xylanase) and other hypothetical proteins including GH3, GH5, GH6, GH7, GH11, GH20, GH32 and GH54. The synergistic action of this enzyme cocktail was assessed by the saccharification of alkali treated wheat straw. Since the Phoma secretome has limited β-glucosidase activity, it was supplemented with commercial β-glucosidase. After supplementation, this enzyme complex resulted in high yields of glucose (177.2 ± 1.0 mg/gds), xylose (209.2 ± 1.5 mg/gds) and arabinose (25.2 ± 0.3 mg/gds). The secretome analysis and biomass hydrolysis by P. exigua revealed its unique potential as a source of hydrolytic enzymes for lignocellulosic biomass hydrolysis.

Keywords
Phoma exigua; Secretome; Glycosyl hydrolase; Biomass hydrolysis
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Unwrapping the hydrolytic system of the phytopathogenic fungus Phoma exigua by secretome analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 10, October 2014, Pages 1630–1636
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us