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Purification, characterization and immunoreactivity of tropomyosin, the allergen in Octopus fangsiao

Paper ID Volume ID Publish Year Pages File Format Full-Text
34508 45030 2014 10 PDF Available
Title
Purification, characterization and immunoreactivity of tropomyosin, the allergen in Octopus fangsiao
Abstract

•The major allergen tropomyosin (TM) of Octopus fangsiao was purified. The purified TM was treated with different food processing.•The digestibility and allergenicity of the treated TM and the native TM were compared.•The food processing for the degrading of allergenicity of octopus TM is proposed.

Octopus is one of the foods that can cause food allergies. In this study, tropomyosin (TM) – the primary allergen in octopus – was purified from Octopus fangsiao following different food processing treatments. The changes in the allergenicity and digestibility of different TM samples were estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunological experiments. The results showed that TM was resistant to pepsin but not to trypsin or α-chymotrypsin. Compared with crude TM, the TM treated by ultrasound-heat (UH) and ultraviolet radiation (UVR) showed no significant change in IgG/IgE-binding ability, except high pressure steaming (HP). And the resistance of the treated TM to digestive enzymes was reduced, with HP-treated TM showing the most significant change in stability. It was different that the resistance of TM to the digestive enzymes was increased after treatment with the Maillard reaction (MR); the IgG/IgE-binding activities of the MR products were almost completely eliminated. Thus, all of these results show that the immunoreactivity of octopus TM can be reduced by HP and MR treatment.

Keywords
Octopus; Allergen; Tropomyosin; Processing; Protease digestion; Immunoreactivity
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Purification, characterization and immunoreactivity of tropomyosin, the allergen in Octopus fangsiao
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 10, October 2014, Pages 1747–1756
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us