fulltext.study @t Gmail

Effects of redox potential control on succinic acid production by engineered Escherichia coli under anaerobic conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
34514 45032 2014 5 PDF Available
Title
Effects of redox potential control on succinic acid production by engineered Escherichia coli under anaerobic conditions
Abstract

•Escherichia coli LL016 showed a high succinic acid productivity in a defined medium.•Oxido-reduction potential (ORP) control was used in succinic acid production.•The effects of ORP control have been investigated in LL016.•The ORP level of −400 mV was preferable in LL016 for succinic acid production.

The effects of oxido-reduction potential (ORP) control on succinic acid production have been investigated in Escherichia coli LL016. In LL016, two CO2 fixation pathways were achieved and NAD+ supply was enhanced by co-expression of heterologous pyruvate carboxylase (PYC) and nicotinic acid phosphoribosyltransferase (NAPRTase). During anaerobic fermentation, cell growth and metabolite distribution were changed with redox potential levels in the range of −200 to −400 mV. From the results, the ORP level of −400 mV was preferable, which resulted in the high succinic acid concentration (28.6 g/L) and high succinic acid productivity (0.33 g/L/h). Meanwhile, the yield of succinic acid at the ORP level of −400 mV was 39% higher than that at the ORP level of −200 mV. In addition, a higher NADH/NAD+ ratio and increased enzyme activities were also achieved by regulating the culture to a more reductive environment, which further enhanced the succinic acid production.

Keywords
Succinic acid; Anaerobic fermentation; NADH/NAD+; ORP
First Page Preview
Effects of redox potential control on succinic acid production by engineered Escherichia coli under anaerobic conditions
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 5, May 2014, Pages 740–744
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us