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Biotransformation pathway and kinetics of the hydrolysis of the 3-O- and 20-O-multi-glucosides of PPD-type ginsenosides by ginsenosidase type I

Paper ID Volume ID Publish Year Pages File Format Full-Text
34524 45032 2014 8 PDF Available
Title
Biotransformation pathway and kinetics of the hydrolysis of the 3-O- and 20-O-multi-glucosides of PPD-type ginsenosides by ginsenosidase type I
Abstract

•Ginsenosidase type I can hydrolyze 3-O and 20-O-multi-glycosides of PPD ginsenoside.•Enzyme hydrolyzed Rb1 and Rb3 with two pathways hydrolyzing 20-O and 3-O-glycosides.•Hydrolyzing 20-O-glycoside speed of Rb1 and Rb3 was faster than that of 3-O-Glc.•Enzyme firstly hydrolyzes 3-O-glucoside of Rb2 and Rc, then hydrolyzes 20-O-glycoside.•Enzyme reaction speed: Rb3 to Rd>Rb1 to Rd>Rc>Rb2>Rd>Rb3 to C-Mx1>Rb1 to Gyp17>F2>Rg3>.

Ginsenosidase type I from Aspergillus niger g.48 can hydrolyze the 3-O- and 20-O-multi-glycosides of PPD-type ginsenosides. The enzyme molecular weight is approximately 74 kDa. When hydrolyzing the glycosides of Rb1, Rb3, Rb2 and Rc, the structures of which only differ in their terminal 20-O-glycosides, ginsenosidase type I hydrolyzes both the 3-O- and 20-O-glycosides of Rb1 and Rb3 using two pathways, but the enzyme first hydrolyzes the 3-O-glucosides of Rb2 and Rc using one pathway. One pathway of Rb1 hydrolyzes the 20-O-Glc of Rb1 to Rd→F2→C-K; another pathway hydrolyzes the 3-O-Glc of Rb1 to Gyp17→Gyp75→C-K. Two hydrolysis pathways are used to hydrolyze the 20-O-Xyl and the 3-O-Glc of Rb3. According to the enzyme reaction parameters Km, Vmax and V0 at a 10 mM substrate concentration, the enzyme hydrolysis velocity values decrease in the following order: the 20-O-Xyl of Rb3→Rd> the 20-O-Glc of Rb1→Rd> the 3-O-Glc of Rc> the 3-O-Glc of Rb2> the 3-O-Glc of Rd> the 3-O-Glc of Rb3→C-Mx1> the 3-O-Glc of Rb1→Gyp17> the 3-O-Glc of F2> the 3-O-Glc of 20(S)-Rg3.

Keywords
Ginsenosidase type I; PPD-type ginsenosides; Biotransformation; Enzyme kinetics
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Biotransformation pathway and kinetics of the hydrolysis of the 3-O- and 20-O-multi-glucosides of PPD-type ginsenosides by ginsenosidase type I
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 5, May 2014, Pages 813–820
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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100% Money Back Guarantee
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