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Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups

Paper ID Volume ID Publish Year Pages File Format Full-Text
34528 45032 2014 5 PDF Available
Title
Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups
Abstract

•The monodisperse magnetic polystyrene microbeads were chosen as the base of support.•The magnetic microbeads is of dispersion and abundant functional groups.•Functionalizable and metal-chelate surfaces for reversible enzyme immobilization.•The regenerated biocatalyst still reserved its original performance.

A novel and simple process for the surface functionalization of micron-sized monodisperse magnetic polystyrene (PS) microbeads was reported. The polystyrene seed particles were prepared prior to the dispersion polymerization method. Afterwards, series of surface chemical modifications on polystyrene microspheres were conducted, and three end-functional microspheres with carboxyl, imidazolyl and sulphydryl groups were obtained. The functional magnetic polystyrene microspheres were prepared by impregnation and subsequent precipitation of ferric and ferrous ions into the polystyrene particles. Finally, the functional magnetic polystyrene was used for the reversible immobilization of glucoamylase via metal-affinity adsorption. The results indicated that the obtained immobilized glucoamylase presented excellent reusability, applicability, magnetic response and regeneration of supports. The magnetic PS microspheres retained >65% of its initial activity at 65 °C over 6 h; and the lowest residual activity of immobilized glucoamylase prepared by regenerated supports still remained about 50% of the initial activity after the 10th cycles.

Keywords
Composite materials; Magnetic materials; Polymers; Microstructure; Electron microscopy
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Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 5, May 2014, Pages 845–849
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us