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Characterization of tubule and monomer derived from VP4 protein of infectious bursal disease virus

Paper ID Volume ID Publish Year Pages File Format Full-Text
34533 45032 2014 8 PDF Available
Title
Characterization of tubule and monomer derived from VP4 protein of infectious bursal disease virus
Abstract

•VP4 protein of infectious bursal disease virus (IBDV) self-cleavages at a VP4 cleavage site in Escherichia coli and forms tubules thereafter.•Deletion of 28 amino acids at the C-terminus of VP4 resulted in both VP4 monomers and dimers instead of tubule formation.•The endopeptidase activity of these VP4 monomers and dimers was about 12.5 times higher than that of VP4 tubules.•Formation of VP4 tubules inhibits VP4 protease activity.

The VP4 protein of infectious bursal disease virus (IBDV) is a serine protease that processes the polyprotein for viral assembly. VP4 has been found to associate primarily with type II IBDV tubules that are 24 nm in diameter. In this study, a chimeric VP4, assigned as HS1VP4, was constructed with a VP4-autocleavage site inserted between the N-terminal His-tag and the VP4 sequence. The results showed that the VP4 forms tubules after the self-cleavage of HS1VP4 when expressed in Escherichia coli. Furthermore, a deletion of 28 amino acids at the C-terminus of VP4 resulted in monomers and dimers instead of tubule formation; mutants of S652A and K692A at active site destroyed the activity. The endopeptidase activity of these monomers and dimers was approximately 12.5 times higher than that of VP4 tubules. Additionally, the formation of tubules inhibited VP4 protease activity, as demonstrated through in vitro assays. The production and characterization of monomers or dimers that have greater endopeptidase activity and protease activity than tubules can provide further insight into VP4 tubule assembly and the regulation of VP4 activity in host cells; this insight will facilitate the development of new anti-IBDV strategies.

Keywords
VP4 protease; Infectious bursal disease virus; Ser/Lys dyad; Birnaviruses; Tubule-like particles
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Characterization of tubule and monomer derived from VP4 protein of infectious bursal disease virus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 5, May 2014, Pages 882–889
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us