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Toward a cost-effective method for α-arbutin production by using immobilized hydroquinone as a glucosyl acceptor

Paper ID Volume ID Publish Year Pages File Format Full-Text
34538 45033 2013 6 PDF Available
Title
Toward a cost-effective method for α-arbutin production by using immobilized hydroquinone as a glucosyl acceptor
Abstract

•The study provided a cost-effective method for α-arbutin production.•H107 resin was used as hydroquinone immobilization medium.•The productivity of α-arbutin enhanced 526% (0.90 g L−1 h−1) in 72 h reaction by using IMHQ as a substrate.•The fermentation broth retained its 78.5% activity after 3 repeated batch reactions.

α-Arbutin is a glycosylated hydroquinone (HQ) which has inhibitory function against tyrosinase. The aim of present study is to develop an efficient and inexpensive method for the production of α-arbutin by using Xanthomonas maltophilia BT-112 as a biocatalyst. HQ, substrate for the bioconversion as glucosyl acceptor, was immobilized on different macroporous resins to reduce its toxic effect on the cells, and the optimal reaction conditions for α-arbutin synthesis were investigated. The research results indicated that H107 resin offered the best adsorption capacity for HQ than other resins. When immobilized hydroquinone (IMHQ) was applied, the maximal HQ tolerance of cells and yield of α-arbutin were 254 mM and 64.7 g L−1 respectively. The α-arbutin productivity (0.90 g L−1 h−1) was 526% higher than that of the control (free HQ) in 72 h reaction and the fermentation broth maintained its full activity for almost three consecutive batch reactions. Additionally, the product was one-step isolated and identified as α-arbutin by 13C NMR and 1H NMR analysis. In conclusion, the results in this work provide a cost-effective method for the production of α-arbutin.

Keywords
α-Arbutin; Xanthomonas maltophilia; Fermentation; Immobilization; Hydroquinone; Macroporous resin
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Toward a cost-effective method for α-arbutin production by using immobilized hydroquinone as a glucosyl acceptor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 10, October 2013, Pages 1447–1452
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us