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Heterologous expression of the osmotolerant yeast Candida glycerolgenesis glycerol-3-phosphate dehydrogenase gene (CgGPD) in Saccharomyces cerevisiae lacking the HOG pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
34541 45033 2013 7 PDF Available
Title
Heterologous expression of the osmotolerant yeast Candida glycerolgenesis glycerol-3-phosphate dehydrogenase gene (CgGPD) in Saccharomyces cerevisiae lacking the HOG pathway
Abstract

•CgGPD expression increased the viability and osmotolerance of gpd1/gpd2 mutant.•CgGPD gave the different inducible expression level with GPD1.•CgGPD and GPD1 enhanced the glycerol production and hyperosmotic stress response.

The cytoplasmic glycerol-3-phosphate dehydrogenase of Candida glycerinogenes encoded by CgGPD is a critical enzyme in overproducing glycerol. The function of CgGPD has been characterized, however little information is known about the participation of CgGPD in the high osmolarity glycerol (HOG) pathway. In this study, expression and the function of CgGPD were investigated in Saccharomyces cerevisiae strains lacking a HOG component (hog1, pbs2 and gpd1/gpd2 mutants). Expression of CgGPD in gpd1/gpd2 mutants not only increased viability but also enhanced the salt tolerance and growth capability in a high osmotic medium. Functional comparison of CgGPD, GPD1 and GPD2 showed that expression of either gene significantly improved growth properties of both hog1 and pbs2 mutants under high osmolarity conditions except that the expression of GPD1 had little effect on the growth of hog1 mutants. Expression of CgGPD and GPD1 led to a similar growth pattern in gpd1/gpd2 mutants however, GPD2 had a perceptible impact on its growth properties. Further analysis showed that intracellular glycerol accumulated increasingly in response to high osmolarity conditions in all mutants expressing CgGPD, GPD1 or GPD2. Furthermore, expression of CgGPD was induced by osmo-stress in hog1 and pbs2 mutants however, the transcriptional strength was correspondingly lower when it was expressed in gpd1/gpd2 mutants.

Keywords
Candida glycerinogenes; Osmotolerant yeasts; Glycerol production; Glycerol-3-phosphate dehydrogenase gene; High osmolarity glycerol pathway
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Heterologous expression of the osmotolerant yeast Candida glycerolgenesis glycerol-3-phosphate dehydrogenase gene (CgGPD) in Saccharomyces cerevisiae lacking the HOG pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 10, October 2013, Pages 1469–1475
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us