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Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura)

Paper ID Volume ID Publish Year Pages File Format Full-Text
34557 45033 2013 11 PDF Available
Title
Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura)
Abstract

•Skin waste of eel fish Evenchelys macrura as a cheap source.•Commercially collagen found non-edible and dumped as the waste of fishes.•It is might can be used as an alternative source of natural mammalian collagen.•Isolated collagen is high thermal stability in comparison to the other skin sources.•It is useful for foods, cosmetics, pharmaceuticals commercial industrials export.

Aim and methodsCollagen is the most abundant protein found in animal body, which is widely used for biomedical and pharmaceutical applications. In the present study, acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin wastes of marine eel fish (Evenchelys macrura) were isolated and characterized.ResultsASC and PSC extracted from eel fish skin showed the yields of 80 and 7.10 percent (based on dry weight), respectively. ASC and PSC comprising different α-chains (α1, α2 and α3) were characterized as type I and exhibited high solubility in acidic pH (1–4) and were soluble in the presence of NaCl at concentration up to 3.0 and 4.0 percent (w/v) for ASC and PSC, respectively. Amino acids analysis of both ASC and PSC contained imino acid of 190 and 200 residues per 1000 residues, respectively. The present results of ASC and PSC from eel fish skin exhibited higher thermal stability of 39 °C and 35 °C, respectively. Similar, Fourier transform infrared (FTIR) spectra of ASC and PSC were observed and suggesting that pepsin hydrolysis did not affect the secondary structure of collagen, especially triple-helical structure.ConclusionThese results suggest that the marine eel fish skin collagen close to the Td (denaturation temperature) of mammalian collagen which could be used in the biomedical materials, food and pharmaceutical industries as an alternative source.

Keywords
ASC, acid-soluble collagen; PSC, pepsin-solubilized collagen; NaCl, sodium chloride; NaOH, sodium hydroxide; Tris–HCl, tris hydrochloride; BSA, bovine serum albumin; βME, β-mercaptoethanol; SDS-PAGE, sodium-dodecyl-sulfate-polyacrylamide gel electrophores
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Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 10, October 2013, Pages 1592–1602
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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