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Extraction and purification of laccase by employing a novel rhamnolipid reversed micellar system

Paper ID Volume ID Publish Year Pages File Format Full-Text
34567 45034 2012 7 PDF Available
Title
Extraction and purification of laccase by employing a novel rhamnolipid reversed micellar system
Abstract

Biosurfactant-based reversed micellar extraction (RME) is an innovative method for separation and purification of biomolecules. In this study, rhamnolipid (RL), a kind of biosurfactant, was firstly adopted to form a novel reversed micellar system for extracting and purifying laccase from Coriolus versicolor crude extract. Several significant factors affecting both forward and backward extraction processes were studied. The appropriate conditions for forward extraction process were: 3.3 mM RL, 50 mM KCl, pH 5.5 and extracting time 40 min. As regards backward extraction process, 250 mM KCl, pH 7.0 and extracting time 40 min were suggested. The corresponding activity recovery (AR) and purification fold (PF) were 92.7% and 4.79, respectively. Electrophoresis analysis indicated that the laccase was successfully purified. After this reversed micellar system was reused three times, the AR and PF declined to 70.8% and 4.35, respectively, indicating that the reversed micellar system could be reused. Comparisons results of synthetic surfactant-based RME and RL-based RME further verified the superiority of RL.

► The biosurfactant rhamnolipid was employed in reversed micellar extraction for purification of laccase. ► Parameters in both forward and backward extraction process were optimized. ► The laccase after extraction was purified 4.79 fold and confirmed by SDS-PAGE profiles. ► The revered micellar system can be reused after backward extraction.

Keywords
Rhamnolipid; Reversed micelle; Reversed micellar extraction; Purification; Laccase
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Extraction and purification of laccase by employing a novel rhamnolipid reversed micellar system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 5, May 2012, Pages 742–748
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us