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A comparative performance evaluation of jute and eggshell matrices to immobilize pancreatic lipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
34568 45034 2012 9 PDF Available
Title
A comparative performance evaluation of jute and eggshell matrices to immobilize pancreatic lipase
Abstract

A pancreatic lipase was immobilized on readily available and inexpensive jute and eggshell matrices. The purity of extracted enzyme was confirmed by SDS-PAGE. The maximum protein load for eggshell was 10.23 mg/g, and for jute, it was 5.7 mg/g. The free enzyme activity retention was greater than 80% for eggshell and 43% for jute. The immobilized lipase was stable over a pH range from 7 to 8 for eggshell and 7.5 to 8.5 for jute with over a temperature range from 25 to 45 °C for eggshell and 37 to 40 °C for the jute. FTIR data indicated new bonds on the jute upon immobilization. Although no new bond was observed, immobilization data on eggshell fit well with the Langmuir adsorption isotherm model. The model constants, Γmax and Kl, were 13.92 mg/g and 0.382 mL/mg, respectively. Mixed adsorption with both ionic and hydrophobic interactions was observed. Lipase adsorption was reduced significantly in presence of Tween 80, whereas the effect was less in case of ionic strength, pH and temperature. For both matrices, scanning electron microscopy (SEM) was used to demonstrate the changes in surface morphology after immobilization. The performance of eggshell was better than that of jute as a matrix for immobilizing pancreatic lipase.

► Jute and eggshell were compared as matrices for lipase immobilization. ► From FTIR study immobilization of lipase on jute was found as covalent interaction. ► Adsorption was validated by Langmuir adsorption isotherm in case of eggshell. ► Stability, effecting parameters and reusability of immobilized lipases were studied. ► Eggshell was found to be better matrix than jute for lipase immobilization.

Keywords
Lipase immobilization; Eggshell; Jute; FTIR; SEM; Adsorption isotherm
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A comparative performance evaluation of jute and eggshell matrices to immobilize pancreatic lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 5, May 2012, Pages 749–757
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us