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Surface modification of iron oxide nanoparticles with polyarginine as a highly positively charged magnetic nano-adsorbent for fast and effective recovery of acid proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
34574 45034 2012 7 PDF Available
Title
Surface modification of iron oxide nanoparticles with polyarginine as a highly positively charged magnetic nano-adsorbent for fast and effective recovery of acid proteins
Abstract

Polyarginine has been successfully bound onto iron oxide nanoparticles via carbodiimide activation as a highly positively charged magnetic nano-adsorbent for protein separation. They were nearly superparamagnetic with a mean diameter of 10.3 ± 2.36 nm, and the binding process did not change the spinel structure of iron oxide. From the analyses of FTIR spectra and zeta potential, the binding of polyarginine on the surface of iron oxide was confirmed and the resultant polyarginine-coated magnetic nanoparticles (PA-MNPs) were positively charged even up to pH 11. By thermogravimetric analysis, the typical product contained about 7.1 wt% of polyarginine. From the adsorption of the proteins with different pI values, the resultant PA-MNPs were found to be quite efficient for the fast and effective adsorption of acid proteins. For the typical acid protein, bovine serum albumin (BSA), the adsorption equilibrium was achieved within few minutes and obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 67.6 mg/g and 0.0623 L/mg, respectively. Moreover, by SDS–polyacrylamide gel electrophoresis, the capability of PA-MNPs for the separation of BSA-lysozyme mixture and egg white was further confirmed. Accordingly, the PA-MNPs were useful for the fast and effective magnetic recovery of acid proteins.

► The surface of iron oxide nanoparticles was modified with polyarginine via covalent binding. ► Polyarginine-coated iron oxide nanoparticles were positively charged in a wide pH range. ► A novel nano-adsorbent was developed for fast and effective magnetic recovery of acid proteins.

Keywords
Polyarginine; Iron oxide nanoparticles; Magnetic recovery; Acid protein
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Surface modification of iron oxide nanoparticles with polyarginine as a highly positively charged magnetic nano-adsorbent for fast and effective recovery of acid proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 5, May 2012, Pages 799–805
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us