fulltext.study @t Gmail

Recombinant α-l-rhamnosidase from Aspergillus terreus in selective trimming of rutin

Paper ID Volume ID Publish Year Pages File Format Full-Text
34578 45034 2012 8 PDF Available
Title
Recombinant α-l-rhamnosidase from Aspergillus terreus in selective trimming of rutin
Abstract

α-l-Rhamnosidase (EC 3.2.1.40) is a biotechnologically important enzyme used for derhamnosylation of many natural compounds. The extracellular α-l-rhamnosidase was purified from the culture of Aspergillus terreus grown on l-rhamnose-rich medium. This enzyme was found to be thermo- and alkali-tolerant, able to operate at 70 °C and pH 8.0. The α-l-rhamnosidase cDNA was cloned from A. terreus, sequenced, and expressed in the yeast Pichia pastoris as a fully functional protein. The recombinant protein was purified to apparent homogeneity and biochemically characterized. Both the native and the recombinant α-l-rhamnosidases catalyzed the conversion of rutin into quercetin-3-glucopyranoside (isoquercitrin), a pharmacologically significant flavonoid usable in nutraceutics. This procedure has high volumetric productivity (up to 300 g/L) and yields the product void of unwanted quercetin. The significant advantage of our expression system consists in shorter production times, up to fourfold increase in enzyme yields and the absence of unwanted β-d-glucosidase as compared to the native production system. Thanks to its unique properties, this enzyme is applicable in a selective synthesis/hydrolysis of various rhamnose containing structures.

► α-l-Rhamnosidase from A. terreus was cloned and expressed in P. pastoris. ► The enzyme is thermo- and alkali-tolerant, operating at 70 °C and pH 8.0. ► Recombinant rhamnosidase was used for production of quercetin-3-glucopyranoside.

Keywords
α-l-Rhamnosidase; Aspergillus terreus; Isoquercitrin; Quercetin-3-β-d-glucopyranoside; Rutin; Pichia pastoris
First Page Preview
Recombinant α-l-rhamnosidase from Aspergillus terreus in selective trimming of rutin
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 5, May 2012, Pages 828–835
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us