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Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus

Paper ID Volume ID Publish Year Pages File Format Full-Text
3458 171 2013 8 PDF Available
Title
Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus
Abstract

Extracellular laccase from the fermentation broth of Pleurotus sapidus was purified using membrane chromatography. Five laccase iso-enzymes produced in submerged cultures were purified; the enzymes had isoelectric points (pI) ranging from 3.3 to 4.7 and a calculated molecular weight of 57.4 kDa. The fermentation broth had a low product titre of 80 μg/l. Hydrophobic interaction (HIC) and ion exchange (IEX) membranes chromatography modules were used in an ÄKTApurifier 100 liquid chromatography system for the dynamic experiments. The pre-treatment of the fermentation broth was not required. The enzyme activity was retained, and enrichment up to 15-fold was observed via activity assays and SDS-Page analysis.

► Laccase from fermentation broth purified using membrane chromatography. ► Membrane chromatography modules with different functionalisation. ► Pre-treatment of the fermentation broth not required. ► Enzyme activity is retained and enrichment up to fifteen fold observed.

Keywords
Membrane chromatography; Membrane adsorption; Downstream processing; Laccase; Pleurotus Sapidus
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Membrane chromatography for the purification of laccase from the supernatant of Pleurotus sapidus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 70, 15 January 2013, Pages 180–187
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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